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Preliminary in silico assessment of the specificity of published molecular assays and design of new assays using the available whole genome sequences of 2019-nCoV

http://virological.org/t/preliminary-in-silico-assessment-of-the-specificity-of-published-molecular-assays-and-design-of-new-assays-using-the-available-whole-genome-sequences-of-2019-ncov/343

Preliminary in silico assessment of the specificity of published molecular assays and design of new assays using the available whole genome sequences of 2019-nCoV

Authors:

Mitchell Holland1, Daniel Negrón1, Shane Mitchell1, Mychal Ivancich1, Katharine W. Jennings1, Bruce Goodwin2, and Shanmuga Sozhamannan2.3

1Noblis, Reston, VA 20191

2Defense Biological Product Assurance Office, Frederick, MD 21702

3Logistics Management Institute, Tysons, VA 22102

Acknowledgement: All the investigators/labs that published the sequence data at GISAID- also see table at the end

Similar analyses/References: Marion Koopmans; Initial assessment of the ability of published coronavirus primers sets to detect the Wuhan coronavirus: http://virological.org/t/initial-assessment-of-the-ability-of-published-coronavirus-primers-sets-to-detect-the-wuhan-coronavirus/321Source 3

WGS: 25 WGS sequences have been downloaded from GISAID; All other data from NCBI BLAST databases (nt, gss, and env_nt - last updated December 2019)

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